SDS-PAGE Study guides, Class notes & Summaries

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...

Biotechnician Assistant Credentialing Practice Exam Latest Update Graded A After performing the PCR reaction with the primers she designed to amplify lanA, the same technician found that her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 kilobase pairs. What is the most likely reason there is an unexpected band in her PCR reaction? She used an annealing temperature that was too low In a biotechnology lab, tap wate...

BACE Exam Practice Test Questions with Complete Solutions What is an example of a product easily found in nature? - ANSWER Antibiotic In a biotechnology lab, what is tap water is best used for? - ANSWER Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - ANSWER mRNA How do DNA molecules vary from one species to another? - ANSWER Number of genes and non-coding regions. What is agar? - ANSWER Solid media on ...

AAMC FL 1 Exam Questions and Answers 2024 If Reaction 2 (Figure 4) is repeated with HCl and the compound shown below, which of the following compounds is NOT a direct product (without rearrangement)? -Answer-Compound A requires a hydride shift to form- therefore not a direct product. NOT an SN2 reaction because of tertiary alcohol- it is SN1 What is the energy of the photons emitted by the LED at a frequency of 610 THz? (Note: h = 6.6 × 10-34 J·s) -Answer-The photon energy is the energy...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light? - Condenser lens The magnification of a microscope is equal to.... - the product of magnification of the ocular times the objective lens The absorbance of UV or visible light by a compound and the subsequent release by that compound of some of the energy as longer wavelength (usually visible) light is known as - fluorescen...

Which of the following is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, tap water is best used for - Answer- washing glassware The order of amino acids on a polypeptide chain is encoded by - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions What is agar? - Answer- Solid media on which bacteria grow What part of an amino acid differs from one to the other? - Answer- R g...

BBMB-301 Test questions and Answers for Final studying (Iowa State) Which class of biopolymers is responsible for separating distinct aqueous compartments within Eukaryotic cells? Lipids The ______ is surrounded by a double membrane and produces approximately 90% of the energy, in the form of ATP, used by a typical living cell Mitochondrion T or F: The central dogma in biochemistry describes the flow of information from RNA to DNA to polypeptide to functional protein False: its DNA to RN...

What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used in SDS-PAGE to denature proteins so they separat...

What is an example of a product easily found in nature? correct answers Antibiotic In a biotechnology lab, what is tap water is best used for? correct answers Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? correct answers mRNA How do DNA molecules vary from one species to another? correct answers Number of genes and non-coding regions. What is agar? correct answers Solid media on which bacteria grow. What part of an amino acid differs from o...