SDS-PAGE Study guides, Class notes & Summaries

Lead Duties: what is your number one priority and name the two categories - Answer- number one priority is to be a role model for the instructors on your team. Two categories are administrative and clinical duties Lead duties: Administrative duties (name and describe all 5) - Answer- 1) upkeep all program materials including the log box and all documents contained in the log box: -service verification forms - program plans (program title, SDs, responses and targets all current and dated) ...

Medical Biochemistry, UCF questions and answers latest top score. Alanine - correct answers.Ala, A Arginine - correct answers.Arg, R Asparagine - correct answers.Asn, N Aspartic Acid - correct answers.Asp, D Experimental techniques: PCR - correct answers.-can amplify small amounts of DNA Experimental techniques: Western blot - correct answers.-measures protein levels in a sample using antibodies Experimental techniques: Southern blot - correct answers.-de...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

FL 5 AAMC MCAT question with complete solution 2023What is the chemical structure of a component found in four of the five cofactors used by Na+-NQR? - correct answer says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has an ubiquinone AN...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS ALL CORRECT Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exc...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer- (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer- 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provid...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

Lead exam questions & answers 2023/2024 Lead Duties: what is your number one priority and name the two categories - ANSWER-number one priority is to be a role model for the instructors on your team. Two categories are administrative and clinical duties Lead duties: Administrative duties (name and describe all 5) - ANSWER-1) upkeep all program materials including the log box and all documents contained in the log box: -service verification forms - program plans (program title, SDs, respo...

What is an example of a product easily found in nature? - Answer-Antibiotic In a biotechnology lab, what is tap water is best used for? - Answer-Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - Answer-mRNA How do DNA molecules vary from one species to another? - Answer-Number of genes and noncoding regions. What is agar? - Answer-Solid media on which bacteria grow. What part of an amino acid differs from one to the other? - Answer-R group How can col...