SDS-PAGE Study guides, Class notes & Summaries

Biochemistry- AAMC FLASH CARDS fully solved 2023The Michaelis-Menten Kinetic equation - correct answer Which combination of lipid type and temperature results in the most disordered membrane? - correct answer Lipid with unsaturated acyl at high tempearture Because unsaturated acyl chains do not pack well well together. They are a mess. When you add high temperature they become a hot mess. Because they is more movement in high temps. The side chain of which amino acid is most likey to f...

FL 5 AAMC MCAT Exam Questions and Answers 100% Verified & Updated What is the chemical structure of a component found in four of the five cofactors used by Na+-NQR? -Answer-says it is composed of flavin structure is a nucleotide base structure B could be structure C also could be structure D is an amino acid down between B and C to help, look at the captioned note on the table. it says Fl represents flavin which is the redox active center in four of the five cofactors of Na+-NQR C has...

WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chainto a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatog...

What is an example of a product easily found in nature? - Answer- Antibiotic In a biotechnology lab, what is tap water is best used for? - Answer- Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? - Answer- mRNA How do DNA molecules vary from one species to another? - Answer- Number of genes and non-coding regions. What is agar? - Answer- Solid media on which bacteria grow. What part of an amino acid differs from one to the other? - Answer- R g...

What is an example of a product easily found in nature? Antibiotic In a biotechnology lab, what is tap water is best used for? Washing glassware. What is the order of amino acids on a polypeptide chain encoded by? mRNA How do DNA molecules vary from one species to another? Number of genes and non-coding regions. What is agar? Solid media on which bacteria grow. What part of an amino acid differs from one to the other? R group How can colorless prot...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? correct answers (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. correct answers 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) diges...

Title: Analysis of the Conservation of S. cerevisiae MET2 Gene in S. pombe Throughout Evolution. Abstract S. cerevisiae and S. pombe are both members of the Ascomycota phylum. The two yeast species diverged from a common ancestor 1 billion years ago. A research project was conducted to investigate the conservation of the S. cerevisiae MET2 gene in S. pombe. The experiments utilized to evaluate MET2 conservation between the two species were spot plating, yeast colony PCR, restriction diges...

Dit deel van de samenvatting "Samenvatting Life Science Bio-informatica Course 5 HAN Nijmegen" bevat de volgende onderwerpen: dialyse, eiwitscheidingstechnieken (1D SDS-PAGE en 2D IEF-SDS-PAGE) en chromatografie (Ion exchange, Size exclusion en Affinity chromatography).

ASCP MB TEST VERIFIED SOLUTIONS 100% PASS What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used i...