Lewis wolpert Study guides, Class notes & Summaries
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11. Small Changes Big Differences
- Class notes • 10 pages • 2020
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- Understand how the neural crest contributes to cardiac development and the pathology underlying congenital cardiac abnormalities.
- Explain how certain SNPs might contribute to congenital abnormalities.
- Discuss the evidence that Notch signalling is important during cardiac morphogenesis
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14. Epigenetic & Mamalian Development
- Class notes • 10 pages • 2020
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- To describe the changes in DNA methylation that occur upon fertilization and during early mammalian development and some of the mechanisms involved
- To understand that TET proteins are involved in active demethylation
- To provide an example of how the epigenome can change post-embryonic development 
- To have been reminded of the method of Chromatin Immunoprecipitation and introduced to Bisulfite Sequencing
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12. Epigenetics Introduction
- Class notes • 10 pages • 2020
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- To have gained an understanding of why epigenetics is relevant to development
- To understand that DNA methylation can impact gene expression and be able to describe an experimental approach to test this
- To explain how patterns of DNA methylation can be maintained through DNA replication
- To understand that covalent histone modifications form a code that can impact gene expression
- To explain how some histone modifications can be maintained through DNA replication
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13. Histone Modifications
- Class notes • 9 pages • 2020
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- Provide an overview of the polycomb and trithorax group complexes and their activities
- Describe evidence that indicates stem cell chromatin is in an open ‘permissive’ state
- Understand what is bivalent chromatin and why it may be important for stem cell biology and development
- Understand the steps of chromatin immunoprecipitation and why it is a useful technique in epigenetic studies
- Describe the Nodal signalling example and experimental approach, and understand how it illustrates ...
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2. Homeotic Genes
- Class notes • 8 pages • 2020
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- Describe experiments that defined compartments of cell lineage restriction.
- Define a compartment and describe the evidence showing that the parasegment is a compartment and the developmentally important unit of the fly.
- Describe genetic experiments demonstrating that HOM-C genes regulate parasegment identity in Drosophila.
- Evaluate whether the vertebrate Hox genes share a conserved function with Drosophila HomC genes
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10. Germ Layer Specification
- Class notes • 8 pages • 2020
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- Discuss the evidence for the nodal morphogen acting during mesoderm induction.
- Discuss the concept of the vertebrate dorsal organiser.
- Discuss the evidence that the brachyury transcription factor is a conserved regulator of germ layer specification.
- Draw conclusions regarding the molecular pathways regulating development of Urbilateria.
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15. Genomic Imprinting
- Class notes • 8 pages • 2020
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- To give an overview of genomic imprinting, when it is initiated and how imprinting disorders can arise
- Describe key events in X-inactivation including the role of the Xist non-coding RNA
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1. Segmentation Genes
- Class notes • 7 pages • 2020
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-Describe the hierarchy of gene activity that regulates Drosophila segmentation
-Distinguish and explain methods used to investigate protein vs mRNA expression
-Describe the syncytial nature of the early stages of fly development and understand its significance.
-Understand and describe at the molecular level how the Gap genes regulate the expression of Eve stripe 2
-Make predictions about segment polarity gene expression in a pair-rule mutant embryo or about pair-rule gene expression in a Gap m...
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8. Building Bodies
- Class notes • 7 pages • 2020
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- Describe the different types of animal body symmetry and the main body axes of bilaterally symmetrical animals.
- Describe the features that define diploblasts versus triploblasts.
- Describe the features that define protostomes versus deuterostomes.
- Understand the importance of the Ediacaran and Cambrian fauna in animal evolution.	
- Describe the common features of phylotypic stage chordate embryos.
- Discuss the evidence indicating that Pax6 is a conserved regulator of eye development. 
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7. Induction Migration of Neural Crest
- Class notes • 7 pages • 2020
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- Explain the significance of the neural crest lineage to vertebrate anatomy.
- Distinguish the ventral-lateral and dorsal pathways of neural crest migration.
- Give examples of cell types derived from specific AP regions of neural crest. 
- Describe and interpret experiments used to ‘fate map’ neural crest cells in avian embryos.
- Describe and interpret experiments used to determine the developmental potential of neural crest cells in avian embryos.
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